Ｏｂｊｅｃｔｉｖｅ：To investigate the loss of histone deacetylase (hdac)9 function during glomerular development.
Ｍｅｔｈｏｄｏｌｏｇｙ：Whole mount in situ hybridization (WISH) was utilized to examine the expression pattern of hdac9 in zebrafish larvae at 24,36,48 and 72 hours postfertilization (hpf) stages; hdac9 mutant zebrafish was generated through CRISPR/Cas9 editing method,ultrastructural changes were observed with transmission electron microscopy (TEM),expression of renal progenitor cells and mature podocytes were examined by WISH.Capped hdac 9 messenger RNA (mRNA) was injected into hdac9 mutant embryos at onecell stage for rescuing the phenotype.
Ｒｅｓｕｌｔｓ：Zebrafish hdac9 started expression in renal progenitor cells and developing glomerulus until its maturation; loss of hdac9 led to glomerular developmental defects,including reduced number of renal progenitor cells and podocytes; Injection of hdac9 mRNA reversed glomerular filtration barrier and podocyte maturation in hdac9 knockout (KO) embryos.
Ｃｏｎｃｌｕｓｉｏｎ：hdac9 is required for normal glomerular development and maturation,probably regulate the transition from renal progenitor cells to podocytes.