关键词: 慢性肾脏病, 非肌性肌球蛋白重链9, 基因多态性

Abstract:

【Abstract】Objective: To explore the association between polymorphisms in MYH9 and Chronic kidney disease in Chinese. Methodology: Five hundred and ninety-five cases, including 301 patients with chronic kidney disease and 294 healthy controls, were enrolled in the this study. Two single nucleotide polymorphisms (SNPs) (rs3752462, rs4821480) were genotyped by TaqMan assay or a restriction fragment length polymorphism assay for a further case–control study. The discrepancies of the patients’ quantitive traits (including age, sex, urinary protein in 24 hour, Serum creatinine, glomerular filtration rate, systolic and diastolic blood pressure, frequency of different primary diseases and frequency of using different kinds of antihypertensive drugs) among different genotypes of the two MYH9 SNPs were analyzed. Results Single factor analysis showed that systolic blood pressure in patients with CT genotype was significantly higher (148±27.4) than that in patients with CC genotype (136±19.1) (P<0.05). Glomerular filtration rate in CC genotype was also significantly higher than that in CT (P<0.05), and TT genotype (P<0.05). After correcting the age factor, Logistic regression indicated that CC genotype was a protective factor of increasing systolic blood pressure. The probability of high blood pressure for CT genotype patients with CKD was 0.175 times that of CC genotype (95%CI：0.071～0.431). Conclusions: The CKD patients who carry the rs3752462 site CC genotype of MYH9 gene are not prone to high blood pressure, and glomerular filtration rate is higher. CC genotype is the independent protective factors of CKD . It may indicate that allele C mutation for T can lead to the increase in systolic blood pressure and even may cause ESRD.

Key words: Chronic kidney disease, MYH9, Gene polymorphism