Abstract:

Ｏｂｊｅｃｔｉｖｅ：To observe the expression  of Krüppellike factor 4 (KLF4) in MES13 cells and to detect the expression  of KLF4,IL6,MCP1in MES13 cells stimulated by high glucose.
Ｍｅｔｈｏｄｏｌｏｇｙ：MES13 cells were stimulated by high glucose(30 mmol/L) for different time.The expression of KLF4 protein was detected by Western blot respectively.MES13 cells were divided into four groups:normal glucose,hypertonic group,high glucose and high glucose with 5AzThe expressions of KLF4,IL6,MCP1 mRNA and protein were detected by RTPCR and Western blot separately.MES13 cells were divided into two groups:LvNC glucose and LvKLF4 group.The expressions of KLF4,IL6,MCP1 mRNA were detected by RTPCR respectively after stimulating by high glucose for 0 hour and 48 hours.
Ｒｅｓｕｌｔｓ：In MES13 cells,the expression of KLF4 protein was decreased after stimulating by high glucose for 24 hours,and it was significantly decreased at 48 hours.The expressions of KLF4 protein and mRNA were significantly decreased in MES13 cells stimulated by high glucose for 48 hours,while the expressions of IL6,MCP1 protein and mRNA were increased obviously.After treatment with 5Az(5 μmol/L),the expressions of KLF4 protein and mRNA were upregulated in MES13 cells,while the expressions of IL6,MCP1 protein and mRNA were downregulated distinctly.After lentivirus plasmid transfected into MES13 cells,the expression level of KLF4 mRNA was significantly increased,while IL6 and MCP1 mRNA expressions didnt change obviously.After stimulating MES13 cells by high glucose for 48 hours,the expression of KLF4 mRNA was significantly decreased in lvnc(hi) group compared with lvnc group,and the expression  of IL6 and MCP1 mRNA were distinctly increased.Compared with lvnc (hi) group,the expression level of KLF4 mRNA was significantly upregulated in LVKLF4(hi) group,while the expression  of IL6 and MCP1 mRNA were downregulated obviously.
Ｃｏｎｃｌｕｓｉｏｎ：KLF4 is expressed in MES13 cells.High glucose can downregulate the expression of KLF4 and upregulate the expressions of IL6、MCP1Low expression of KLF4 is related with inflammation in MES13 cells stimulated by high glucose.Upregulate KLF4 expression can alleviate cell inflammation.Its mechanism probably is associated with DNA methylation partly.

Key words: diabetic kidney disease, Krüppel-like factor 4, inflammation, 5-azacytidine