关键词: 腹膜透析液, 腹膜间皮细胞, 细胞凋亡, 腹膜纤维化

Abstract:

Ｏｂｊｅｃｔｉｖｅ：To compare the influence of new  polysaccharide bicarbonate peritoneal dialysis fluids (PDF) on the human peritoneal mesothelial cells (HPMC) apoptosis and interstitial fibrosis factors.
Ｍｅｔｈｏｄｏｌｏｇｙ：Human peritoneal mesothelial cells (HPMC) were cultured in vitro，then treated with the new PDF and traditional PDF with different concentration of glucose (15%,25%,425%). Realtime quantitative PCR was used to detect the mRNA expression of apoptotic factors Bax,Caspase3 and apoptosis inhibition factor Bcl2,TGFβ1 and FN. ELISA determined the secretion of TGFβ1 expression,western blot was used to detect apoptosis protein Bax,Caspase3,apoptosis inhibition proteins Bcl2 and FN.
Ｒｅｓｕｌｔｓ：After a certain time of  stimulation on HPMC,the higher the  glucose concentration (15%、25%、425%) of traditional PDF,Bax,Caspase3,TGFβ1,FN increased more significantly,apoptosis inhibition factor Bcl2 decreased more significantly. After  stimulation with 25%,425% PDF,fibrosis related factor TGFβ1 and FN were much higher than that of the negative control group and new PDF group (P<005).
Ｃｏｎｃｌｕｓｉｏｎ：The new  polysaccharide bicarbonate peritoneal dialysis fluid is superior to the traditional peritoneal dialysis fluid,its effect on the apoptosis protein of peritoneal mesothelial cells is weaker than that of the traditional peritoneal dialysis fluid,peritoneal mesothelial cell fibrosis degree is aslo lower,which has good prospects for clinical application