关键词: 肠道屏障, 尿毒症, 微炎症, 细菌移位

Abstract: Ｏｂｊｅｃｔｉｖｅ：To evaluate the status of intestinal barrier function and to explore the relationship between intestinal barrier dysfunction and microinflammation in  uremic rats.
Ｍｅｔｈｏｄｏｌｏｇｙ：SD rats were randomly divided into uremic group and sham operation group.The uremic model was established by 5/6 nephrectomy.Only renal capsule were opened in sham operation group.The green fluorescent proteinlabeled tracer was constructed and fed to two groups of rats.The green fluorescent proteinspecific gene fragment was amplified from the extraintestinal tissues such as liver,spleen,mesenteric lymph node and peripheral blood by PCR to detect the presence of intestinal bacteria were transferred to the extraintestinal tissue site; HE staining was used to observe pathological changes of intestinal tissue,transmission electron microscopy was used to observe ultrastructural changes.Expressions of JAM1,claudin1 and Occludin in intestinal tissues were observed by immunohistochemistry.Serum hsCRP,IL6 and TNFα levels were detected.
Ｒｅｓｕｌｔｓ：The intestinal bacterial translocation rate  was significantly higher in uremic group (70% vs 10%,P<005).The ultrastructure showed that the tight junction structure between intestinal epithelial cells in uremic group was incomplete and electron density decreased.The microvilli were disorganized,partially broken and disappeared.Serum hsCRP (603±170 vs 345±126),IL6 (3762±321 vs 1981±655),and TNFα (2848±1085 vs 1733±572) were significantly higher in uremic group than those in shamoperated group. (P<005).
Ｃｏｎｃｌｕｓｉｏｎ：
Intestinal mucosal barrier dysfunction exists in experimental uremia.Intestinal bacteria can translocate to distant organs and blood circulation,then activate inflammatory response,which may be one of the mechanisms of microinflammation.

Key words: intestinal barrier, uremia, microinflammation, bacterial translocation