关键词: Klotho蛋白, 缺血-再灌注肾损伤, 氧化应激

Abstract:

Ｏｂｊｅｃｔｉｖｅ：To research the effect of Klotho protein on oxidative stress in ischemiareperfusion renal injury rats,and regulating of nitric oxide (NO) synthesis.
Ｍｅｔｈｏｄｏｌｏｇｙ：One hundred twenty male Wistar rats were randomly divided into NOR+NS group,Sham+NS group,Sham+Klotho group,I/R+NS group and I/R+Klotho group. After making models of acute ischemiareperfusion kidney injury，six rats were put to death separately at 1 hour,5 hours,12 hours,and 24 hours after operation per group. Morphological changes in the kidney were observed by HE staining，plasma levels of NO,Tissue levels of peroxidase (MPO) and  superoxide dismutase (SOD) in kidney were detected by colorimetric method，Klotho protein were detected by the ELISE method.
Ｒｅｓｕｌｔｓ：24 hours after ischemiareperfusion injury,I/R+NS group rats had more vacuolar degeneration of renal tubular epithelial cells,however  I/R+Klotho group rats had little tubular epithelial changes. In I/R+NS group,expression of Klotho protein, NO and SOD activities was significantly lower than other groups, also MPO,Scr and BUN were significantly higher than other groups. In I/R+Klotho group, the levels of NO,MPO,SOD,Scr and BUN were improved compared with I/R+NS group (P<005).
Ｃｏｎｃｌｕｓｉｏｎ：Klotho protein may alleviate renal ischemiareperfusion injury by regulating NO synthesis against oxidative stress.

Key words: Klotho protein, ischemia-reperfusion renal injury, oxidative stress