Abstract: Ｏｂｊｅｃｔｉｖｅ：To analyze the intestinal flora structure of patients with lupus nephritis (LN) by illumina high-throughput sequencing technology.
Ｍｅｔｈｏｄｏｌｏｇｙ：A total of 60 patients with LN (LN Group, LG group) who were admitted to the Fourth Hospital of Hebei Medical University from January 2022 to December 2023 and 60 healthy people who underwent physical examination during the same period (control group) were selected as the study objects. Fecal samples of all subjects were collected. 16S ribosomal RNA (16S rRNA) was used to analyze the intestinal flora structure and diversity of the two groups of subjects.
Ｒｅｓｕｌｔｓ：There were no significant differences in age, gender, BMI, blood lipid and fasting blood glucose between LG group and control group (P>0.05). The results of β-diversity analysis suggested that there were significant differences in the composition of microflora between the two groups. At the gate level, the abundance of Betularia (WPS-2) in LG group was significantly higher than that in control group. At the generic level, there were significant differences between LG group and control group. They mainly include Eubacterium xylanophilum_group, Tyzzerella_4, Erysipelatoclostridium, Lysobacter and Holman Holdemanella, Prevotella, Anaeromyxobacter, Acidibacter, Bergeyella and Flavisolibacter, etc.
Ｃｏｎｃｌｕｓｉｏｎ：There were significant differences in the intestinal flora characteristics and structural composition of healthy people in patients with lupus nephritis. The intestinal flora of patients with lupus nephritis had a higher abundance of Betulae (WPS-2), and the levels of several genera were also significantly different compared with healthy people. These results suggest that a variety of microbial communities, such as Tyzzerella_4 and Clostridium erythematosus, play an important role in the occurrence and progression of lupus nephritis. Different microbial communities may provide new clues for the diagnosis and treatment of LN, and provide important references for future clinical research and treatment strategies.

Key words: lupus nephritis, intestinal microbiota, high-throughput sequencing, 16S ribosomal RNA