关键词: 长链非编码RNA, lncRNA-Gm4419miR-7214-3p, 肾小球系膜细胞

Abstract: Ｏｂｊｅｃｔｉｖｅ：To investigate the effects of lncRNA-Gm4419 binding with miR-7214-3p on inflammation, fibrosis, and proliferation in mouse glomerular mesangial cells (MGMCs) under high glucose conditions.
Ｍｅｔｈｏｄｏｌｏｇｙ：The expression of miR-7214-3p in MGMCs cultured in low glucose (5.5mmol/L glucose + 19.5mmol/L mannitol) and high glucose (25mmol/L glucose) conditions was measured using qRT-PCR. The binding site of lncRNA-Gm4419 to miR-7214-3p was predicted by bioinformatics analysis. The binding ability of lncRNA-Gm4419 to miR-7214-3p in MGMCs was determined using the Luciferase reporter gene method. Additionally, the expression of monocyte chemotactic protein 1 (MCP-1), tumor necrosis factor (TNF-α), interleukin-1 beta (IL-1β), fibronectin (Fn), and type Ⅳ collagen (Col.Ⅳ) was assessed by qRT-PCR, and cell proliferation was evaluated using flow cytometry and Cell Counting Kit-8 (CCK8) assays.
Ｒｅｓｕｌｔｓ：MiR-7214-3p expression significantly decreased in MGMCs cultured in high glucose. In the low glucose group, silencing miR-7214-3p led to increased expression of MCP-1, TNF-α, IL-1β, Fn, and Col.Ⅳ, as well as enhanced cell proliferation. Conversely, in the high glucose group, overexpression of miR-7214-3p resulted in decreased expression of MCP-1, TNF-α, IL-1β, Fn, and Col.Ⅳ, as well as decreased cell proliferation. Furthermore, overexpression of lncRNA-Gm4419 in MGMCs led to increased expression of MCP-1, TNF-α, IL-1β, Fn, and Col.Ⅳ, and enhanced cell proliferation. However compared to overexpression of lncRNA-Gm4419 alone, when both lncRNA-Gm4419 and miR-7214-3p were overexpressed, the expression of MCP-1, TNF-α, IL-1β, Fn, and Col.Ⅳ decreased, along with reduced cell proliferation.
Ｃｏｎｃｌｕｓｉｏｎ：Under high glucose conditions, lncRNA-Gm4419 promote the inflammatory response, fibrosis, and proliferation of MGMCs by inhibiting the expression of miR-7214-3p.

Key words: long non-coding RNA, lncRNA-Gm4419, miR-72143p, glomerular mesangial cells