ISSN 1006-298X      CN 32-1425/R

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肾脏病与透析肾移植杂志 ›› 2011, Vol. 20 ›› Issue (4): 397-400.

• 论文 • 上一篇    

低黏度包埋剂在肾活检电镜标本制备中的应用

  

  • 出版日期:2011-08-31 发布日期:2011-09-01

Low viscosity embedding medium Eponate 12 in electron microscopy of renal biopsy

  • Online:2011-08-31 Published:2011-09-01

摘要:

目的:探讨低黏度包埋剂Eponate 12树脂在肾活检电镜技术中的应用。  方法:选取40例肾活检穿刺标本,分为实验组和对照组各20例,分别采用低黏度包埋剂Eponate 12和Epon 812常用包埋剂包埋处理标本,观察不同包埋剂及处理方法对肾活检电镜标本结果的影响。  结果:低黏度包埋剂Eponate 12聚合时间短,全程标本处理耗时较传统包埋剂Epon 812缩短48~60h,组织、细胞超微结构保存良好,超薄切片的染色性能和图像反差佳。  结论:使用低黏度包埋剂Eponate 12在保证切片质量的同时明显缩短制片时间,在肾活检电镜技术中不失为一良好的选择,能较好应用于肾活检电镜技术中。

Abstract:

Objective: To investigate the application of low viscosity resin embedding medium Eponate 12 in electron microscopy of renal biopsy.   Methodology: 40 cases of biopsy specimens divided into experimental and control group, 20 cases were low-viscosity embedding medium Eponate 12 and Epon-812 embedding medium commonly used method for embedding specimen processing. Observe the impact on the results in the different agents and treatment methods of embedding.  Results: The low viscosity embedding medium Eponate 12 polymerization time is short, the entire sample preparation time-consuming than conventional Epon 812 embedding medium shortened 48-60h, tissues and cell ultrastructure well-preserved, ultrathin sections stained a good performance and image contrast.  Conclusion: The using of low viscosity embedding medium Eponate 12 ensuring the quality of production while significantly reducing the time of sample preparation can be better used in electron microscopy of renal biopsy.