Ｏｂｊｅｃｔｉｖｅ：To explore the mechanism of necroptosis in lupus nephritis and the efficacy of receptor interaction protein kinase 1 (RIPK1) inhibitor necrostatin1(Nec1) in the treatment of lupus nephritis.
Ｍｅｔｈｏｄｏｌｏｇｙ：MRL／lpr mice at the age of 19 weeks was injected intraperitoneally with Nec1 [35 mg／(kg·d)] for 3 weeks. Changes were detected in serum creatinine, complement levels and urine protein/creatinine ratio. Kidney pathological staining was used to observe kidney pathological changes and the changes in expression of necroptosisrelated proteins. In vitro,the expression of necroptosisrelated molecules in mesangial cells treated with Nec1 were measured. Changes in the expression of proinflammatory factors were detected by qRTPCR.
Ｒｅｓｕｌｔｓ：The systemic immune inflammatory response and kidney damage of mice with lupus nephritis were alleviated after treatment with Nec1,serum antinuclear antibodies titers,antidoublestranded DNA antibody titers,and urine protein/creatinine ratio were decreased,and Nec1 inhibited mesangial cell necrosis and the release of proinflammatory factors in vitro.
Ｃｏｎｃｌｕｓｉｏｎ：RIPK1 inhibitor Nec1 can inhibit the necrotizing apoptosis of mesangial cells and relieve the symptoms of lupus nephritis.