Abstract:

Ｏｂｊｅｃｔｉｖｅ：To investigated the involvement of macrophages in bacterial translocation(BT) and microinflammation in uremic rats and whether Lactobacillus(LB) can influence macrophage activity.
Ｍｅｔｈｏｄｏｌｏｇｙ：Macrophages and GFPlabeled tracer bacteria in intestinal and parenteral tissues were observed by fluorescence microscopy. To detect changes after adminstration of  LB in the level of intestinal permeability,blood endotoxin,hsCRP,IL6,TNFα. RTPCR and western blot were employed to assess the mRNA and protein expression of EGR1 and TLR4.
Ｒｅｓｕｌｔｓ：In uremic rats,the colocalization of GFPlabeled tracer bacteria and macrophages was visible in intestinal and parenteral tissues. Administration of Lactobacillus LB restored the protrusions and pseudopodia. Compared with the uremia group,the uremia+probiotic group exhibited macrophages with lower mRNA and protein expression of TLR4 and EGR1 (P<005) and endotoxin,intestinal permeability,hsCRP,IL6 and TNFα were significantly lower than those in uremia group (P＜005).
Ｃｏｎｃｌｕｓｉｏｎ：
Macrophages with impaired phagocytic function are associated with BT.

Key words: macrophage, bacterial translocation, microinflammatio, nurem