Abstract:

Ｏｂｊｅｃｔｉｖｅ：To investigate the mechanism of Tcellderived extracellular vesicles (EVs) caused podocyte injury in FSGS and the injuryinducing miRNA.
Ｍｅｔｈｏｄｏｌｏｇｙ：EVs were isolated from biopsyproven FSGS patients and healthy controls plasma.MiRNAs differentially expressed in FSGS EVs were analyzed by chip arrays and RTPCR.Thirty milligrams EVs were injected into 8weekold male Balb/c mice through tail vein every other day.Urine albumin/creatinie was measured and kidney structure was analyzed after proteinuria occurred.EVs were also added to culture medium of podocytes.Podocyte cytoskeleton was measured.Jurkat cells were transfected with target miRNA to gain target miRNArich EVs.These EVs were injected into mice and added to cultured podocytes.
Ｒｅｓｕｌｔｓ：MiR193a is elevated in EVs from FSGS patients.EVs induced podocyte injury through miR193a induced down regulation of WT1.
Ｃｏｎｃｌｕｓｉｏｎ：Our results demonstrate a novel mechanism by which EVs induced podocyte injury through miR193a.

Key words: focal segmental glomerulosclerosis, extracellular vesicles, microRNA