Abstract:

Ｏｂｊｅｃｔｉｖｅ：To investigate the effects of various concentrations of citrate on cytokines release by monocyte under stimulation of lipopolysaccharide(LPS) in vitro.
Ｍｅｔｈｏｄｏｌｏｇｙ：Human acute monocyte leukemia cell line(THP1) cells were cultured in vitro with different concentrations of trisodium citrate (TSC,0,05,10 mmol/L),and were stimulated with different concentrations of LPS(0,005,01,1 μg/ml).Cell samples and supernatants were collected 6h,12h,24h and 48h after cell culture.The monocyte viability,concentrations of interleukine6(IL6),tumor necrosis factorα (TNFα) and ionized calcium(Ca2+) were observed.
Ｒｅｓｕｌｔｓ：Various concentrations of citrate did not inhibit the viability of monocyte (P>005).The monocyte TNFα production after LPS stimulation was augmented with 05 mmol/L and 1 mmol/l citrate at 12h and 24h when compared with no citrate(P<001).The production of IL6 was augmented with 05 mmol/L citrate at 24h and 48h(P<005)，but was inhibited with 10 mmol/L citrate when compared with no citrate (P<001).
Ｃｏｎｃｌｕｓｉｏｎ：
Citrate in the investigated concentrations has no cytotoxicity to monocyte.After stimulation by LPS,production of TNFα was augmented with citrate,while production of IL6  was augmented only with low concentration of citrate but was inhibited with high concentration of citrate.

Key words: citrate concentration, monocyte, cytokine