Abstract:

Abstract   Objective: To examined the microRNA expression profile of urine supernatant in patients with focal segmental glomerulosclerosis (FSGS) and analyze the correlation between the level of miRNAs and the activity of disease. Methodology: Urine supernatant samples from patients with active FSGS (urinary protein excretion ≥3.5g/24h), non-active FSGS (urinary protein excretion ≤0.4g/24h) and normal controls were tested by quantitative Real-time PCR. The correlation between the urinary miRNAs expression levels and clinical indexes were analyzed. In order to evaluate the specificity, urinary miRNAs level of patients with membranous nephropathy (MN) and diabetic nephropathy (DN) was also detected for validation. Results: The urinary expression levels of miR-196a, miR-30a, miR-155 and miR-490 were significantly elevated in active FSGS compared to the non-active FSGS patients and normal control (P<0.001). The expression level of miR-196a, miR-30a, miR-155 and miR-490 was correlated with clinical indexes, such as proteinuria and serum albumin level (P<0.05). However, the same change of miR-196a, miR-30a and miR-490 was not found in the urine of MN and DN patients. Conclusion: Urinary expression level of miR-196a, miR-30a and miR-490 correlated with disease activity suggested that miR-196a, miR-30a and miR-490 might be the new urinary biomarker for disease activity and therapeutic effects of FSGS.

Key words: KEY WORDS：FSGS     ,  urinary microRNAs    , biomarker.