Abstract: Ｏｂｊｅｃｔｉｖｅ：To investigate the correlation between biomarkers in renal lavage fluid from cardiac death organ donation (DCD) donors and delayed renal graft function (DGF) after kidney transplantation, and to explore its predictive value for the occurrence of DGF.
Ｍｅｔｈｏｄｏｌｏｇｙ：142 kidney transplant patients admitted to our hospital were selected as the research subjects, and were divided into the DGF group (n=18) and the immediate recovery of transplanted kidney function (IGF) group (n=124) based on whether they experienced DGF after transplantation. Compare the concentrations of biomarkers such as lipid transport protein (NGAL), interleukin-18 (IL-18), liver type fatty acid binding protein (L-FABP), and kidney injury molecule-1 (KIM-1) in two groups of renal lavage fluid, and analyze their correlation with the occurrence of DGF.
Ｒｅｓｕｌｔｓ：The concentrations of IL-18, KIM-1, and L-FABP in the DGF group showed relatively small differences compared to the IGF group (P>0.05); The NGAL concentration in the DGF group was significantly higher than that in the IGF group, with a significant difference (P<0.05). The blood creatinine levels in the DGF group were significantly higher than those in the IGF group on the 1st, 2nd, 3rd, and 7th day after surgery (P<0.05). The receiver operating characteristic (ROC) curve showed that when the Biomarker NGAL and KIM-1 jointly predicted DGF, the diagnostic value was the highest (AUC=0.931, P<0.001, sensitivity=100％, specificity=73.8％). Further analysis found that when NGAL+KIM-1+was used to detect the blood creatinine value on the 3rd day after surgery, the accuracy was high, the sensitivity could reach 100％, the AUC could reach 0974, the specificity was 86.3％, and P<0.001.
Ｃｏｎｃｌｕｓｉｏｎ：The biomarker NGAL in donor renal lavage fluid is positively correlated with delayed recovery of renal function in kidney transplant patients. The diagnostic value of using NGAL+KIM-1+blood creatinine value on the 3rd day after surgery to predict the occurrence of delayed recovery of renal function is high, which can provide guidance for the evaluation of donor kidney quality and subsequent treatment.

Key words: organ donation in cardiac death, biomarkers of renal lavage fluid, delayed renal graft function