Abstract:

ABSTRACT： Objective: To evaluate the role of cell apoptosis imbalance and Fen1 gene mutation in the pathogenesis of lupus nephritis. Methodology: (1) Thirty paraffin organizations from renal biopsy of patients with LN was used TUNEL method to detect the cell apoptosis, the relation of LN pathology classification, SLE activity index 2000 (SLEDAI 2000) and kidney tissue cell apoptosis index (AI) was discussed.(2) Fen1 gene 61563142~61563342 were analyzed by extraction of genomic DNA from the peripheral blood of 50 LN patients、25 healthy individuals and 20 patients with cancer of the stomach. Results: (1) The diffused apoptosis was identified in all samples of 30 LN patients (LN Ⅳ40.0%, LNⅡ26. 7%, LN Ⅲ16. 7%, LNⅤ0.13% and LN type 0 01 %).  The correlation analysis showed that SLEDAI was significantly correlated with apoptosis index (0 < R < 1, P < 0.05). (2) Fen1 gene 61563142~61563342 was not significant difference among LN patients, healthy controls and patients with stomachcancer. Conclusion: Our data suggested that the cell apoptosis dysfunction play a key role in the pathogenesis of LN although Fen1 gene 61563142~61563342 was not found significant difference in LN patients. The larger sample or Genome-Wide Association Studies should be needed.

Key words: Fen1 gene variants   , lupus nephritis  , apoptosis