Abstract:

Objective: To investigate the application of low viscosity resin embedding medium Eponate 12 in electron microscopy of renal biopsy.   Methodology: 40 cases of biopsy specimens divided into experimental and control group, 20 cases were low-viscosity embedding medium Eponate 12 and Epon-812 embedding medium commonly used method for embedding specimen processing. Observe the impact on the results in the different agents and treatment methods of embedding.  Results: The low viscosity embedding medium Eponate 12 polymerization time is short, the entire sample preparation time-consuming than conventional Epon 812 embedding medium shortened 48-60h, tissues and cell ultrastructure well-preserved, ultrathin sections stained a good performance and image contrast.  Conclusion: The using of low viscosity embedding medium Eponate 12 ensuring the quality of production while significantly reducing the time of sample preparation can be better used in electron microscopy of renal biopsy.