Abstract:

Ｏｂｊｅｃｔｉｖｅ：To determine the effect of Salvia Przewalskii extract (SPE) on Puromycin Aminonucleoside (PAN)induced rat podocytes injury.
Ｍｅｔｈｏｄｏｌｏｇｙ：The rats were divided into six groups, and each group was 12 rats. They were treated with either PAN only or PAN followed by tacrolimus or low, medium, highdose SPE and normal control. The effects of SPE on podocyte injury 5, 10, 15 and 21 days following treatment were evaluated.
Ｒｅｓｕｌｔｓ：1) Proteinuria was observed starting on day 5 except in normal control group. The peak levels of proteinuria were different among the groups with tacrolimus, and highdose SPE significantly decreased proteinuria relative to the PAN and low and mediumdose SPE groups. The proteinuria in each group was decreased after 15 days and returned to a normal level after 21 days. 2) With electron microscopy, the foot process effacement was observed starting on day 5, but that was lighter in the tacrolimus and highdose SPE groups. 3) In IHC staining, the increasing expressions and better linear distributions of nephrin and podocin were revealed in the highdose SPE and tacrolimus groups. Those results were also confirmed by Western blot analysis. 4) The mRNA levels of nephrin and podocin in the tacrolimus and highdose SPE groups were significantly higher than that of the other groups on day 5, 10, and 15.
Ｃｏｎｃｌｕｓｉｏｎ：SPE can protect podocytes from PANinduced injury.