Abstract:

ABSTRACT Objective: To explore kidney disease-related proteins and kidney damage-related proteins in urine of patients with kidney disease. Methodology: Urine proteomic data of patients with kidney disease came from urine proteomic profiles of FSGS and MN with nephrotic syndrome. Plasma, urine and kidney proteomic data of healthy subjects were obtained from PeptideAtlas2013. The data from urine proteomic of patients with kidney disease were compared to the data from plasma, urine and kidney proteomic of healthy subjects. The proteins which enriched in urine proteomic of patients with kidney disease to urine proteomic of healthy subjects were named by kidney disease-related protein. Coincidence analysis for above four proteomics was made to search kidney damage-related proteins which are not in plasma proteomic of healthy subjects, shared in urine proteomic of patients with kidney disease and kidney proteomic of healthy subjects, and enriched in urine proteomic of patients with kidney disease to urine proteomic of healthy subjects. Results: A total of 40 kidney disease-related proteins and 4 kidney damage-related proteins, Ubiquitin-60S ribosomal protein L40, S-phase kinase-associated protein 1, TGF-beta receptor type-2 and Glutathione S-transferase A3 was found. Conclusion: By comparing data from above four proteomic, 40 kidney disease-related proteins and 4 kidney damage-related proteins were achieved from urine of patients with kidney disease. These proteins need to be further determined.

Key words: kidney diseases, urine, proteomic biomarker