Abstract:

Abstract: Objective:Ⅳ collagen α5 antibody hereditary nephrotic syndrome Alport play a very important role, in many renal centers for immunofluorescence staining skin differential diagnosis of hereditary nephropathy. Currently, there are two commercially available antibody reagents, Wieslab company is certified diagnostic kits, in frozen sections using a two-step indirect immunofluorescence staining, Cosmo BioUSA is research reagents in the frozen sections using a step direct immunofluorescence fluorescence staining. The aims of this paper is to compare the different organizational units of antibodies, and the differences between the different methods. Can research reagents for clinical diagnosis. Methodology: January 2013 to March were 26 cases of frozen tissue biopsy and 15 cases of paraffin skin tissue, the use of two antibodies parallel comparison companies. Monoclonal mouse anti-human collagen type Ⅳ α5 antibody (Wieslab clone ALP105) by indirect immunofluorescence, monoclonal mouse anti-human collagen type Ⅳ α5 antibody (Cosmo BioUSA clone CFT-45325) using direct immunofluorescence. Results: The two randomized, blinded pathologist look for kidney frozen tissue and found two kinds of antibody staining caused no difference in the results for the diagnosis, only a small amount of specimen staining intensity difference. For skin paraffin tissue, two kinds of staining was found quite different. Conclusion: Cosmo BioUSA company antibodies using the step-saving, and the cost is relatively low. Kidney frozen tissue staining can replace Wieslab company certified diagnostic kits. The skin should choose Wieslab companies paraffin tissue kit.

Key words: Alport syndrome, collagen type Ⅳ, hereditary disease, immunofluorescence