Abstract:

ABSTRACT   Objective: To observe the influence of uremia, peritoneal dialysis on rat peritoneal charge barrier and the correlation between charge barrier and albumin loss from peritoneal dialysate, and to investigate the role of heparin on charge barrier and albumin loss.  Methodology: Forty male SD rats were randomly divided into control group (sham operated group，n=10)，uremia group (5／6 nephrectomy，n=10) , uremia with PD group (n=10) and heparin with PD group (n=10) . Study was carried out 4 weeks and blood sample and peritoneal fluid were collected, and then peritoneal charge barrier was measured by differential clearance of isoamylase (Cpam/Csam). Protein loss of peritoneal fluid was also determined.  Result: （1）Cpam/Csam of control group, uremia group, PD group and heparin group were 1.91±0.89, 2.32±0.74 , 3.11±0.76 and 2.24±0.59 respectively. Figure of the three former were higher than control group significantly (P＜0.01 all) and there was also significantly difference between PD group and Heparin group, while no difference between uremia group and PD group. (2) Albumin loss of peritoneum in uremia group, PD group and Heparin group were significantly higher than that in control group (41.3±8.34 mg, 49.2±3.61 mg and 39.9±3.73 mg vs 27.1±5.66 mg, P＜0.01 all ). At the same time, the figure in PD group was remarkably higher than that in uremia group, P＜0.05. On the contrary, the figure in heparin  group was significantly lower than that in PD group. (3) Cpam/Csam was negative correlated with protein loss (r=-0.469, P＜0.01).  Conclusion: Charge barrier of peritoneum could be damaged at uremic state, and peritoneal dialysis may do harm to charge barrier further, however, heparin could improve charge barrier status and reduce the loss of albumin.

Key words: 【key words】SD rats, Continuous ambulatory peritoneal dialysis, peritoneum, charge barrier, amylase, heparin