Abstract:

ABSTRACT    With the development of translational medicine, we realize that systems biology solve complex biological problems (i.e. macro issues, sophisticated programs, and solution the problem). At the same time, we have a more understanding in the real meaning of life. For example, the early medical researches focus on the gene, and developed genomics. But in the end of the Human Genome Project, the scientists found the transcriptional level changes which do not change phenotypic. Phosphoproteomics is a branch of proteomics that identifies, catalogs, and characterizes proteins containing a phosphate group as a post-translational modification. Phosphorylation is a key reversible modification that regulates protein function, subcellular localization, complex formation, degradation of proteins and therefore cell signalling networks. Quantitative methods can assess the relative change in phosphorylation for several thousand sites in a single experiment, detect novel phosphorylation sites, study dynamic phosphorylation events, understand cell signaling pathways and discovery the new drug target[1]. Here, the phosphoproteomics apply in the life sciences, introduce the characteristics and defect of phosphoproteomics are reviewed.

Key words: Key words： phosphoproteomics , life science , pharmacy , medicine , nephrology