Abstract:

ABSTRACT  Objective：To observing the effect of tripterygium wilfordii polyglucosides on the expression of transforming growth factor-β1 (TGF-β1) 、bone morphogenetic protein (BMP-7) and Gremlin in renal tissue of diabetic nephropathy rats, to explain the mechanism of triptergium wilfordii polyglucoside delay renal interstitial fibrosis. Methodology： Forty Sprague – Dawlay (SD) rats were randomly divided into normal control group, diabetes mellitus control group (group DN) and triptergium wilfordii polyglucoside treated diabetes mellitus group (group TWP).  Diabetes mellitus were induced by high sugar and fat diet and streptozocin(STZ), and groupTWP were treated with triptergium wilfordii polyglucoside（50mg/Kg•d）. The weight of rat、24 hours protein (24 h U - TP) and urine NAG were examined in 2th 、4th、8th and 16th week. After 16 weeks, blood glucose(GLU) , serum creatinine (Scr ), blood urea nitrogen(BUN), kidney hypertrohy index were measured. Partial nephridial tissue were observed HE staining. Mean glomerular area and mean glomerular volume were determined. RT-PCR，western blotting，immunohistochemistry were used to analyse TGF-β1 、BMP-7 and Gremlin gene and protein expression in kidney tissue of three group rats.  Results：Compared with the untreated diabetic group, urinary albumin excretion, serum creatinine and blood urea nitrogen of triptergium wilfordii polyglucoside treated rats were substanitially decreased. The expression of TGF-ß1 and Gremlin were lower and BMP-7 were upper in triptergium wilfordii polyglucoside treated group. Conclusions：Triptergium wilfordii polyglucoside has protective effect on renal interstitial fibrosis in diabetic rats, partly through down-regulating TGF-ß1、Gremlin and up-regulating BMP-7.

Key words: triptergium wilfordii polyglucoside   , diabetic nephropathy  , renal interstitial fibrosis  , TGF-ß1  , Gremlin , BMP-7