Abstract:

Objective: To explore the protective effect and the mechanism of Cordyceps extract on renal tissue in rats with ischemia reperfusion injury. Methodology: The kidneys of seventy-two male Sprague-Dawley rats were randomly divided into 3 groups: Saline group, kidney preservation solution (HCA group), and artificial Cordyceps Liquid Composite Group (CS-HCA group). We observed the degree of tubulo-interstitial lesions, apoptosis index (AI) of the renal tubular epithelial cell and superoxide dismutase (SOD) activity and concentration of malondialdehyde aldehyde (MDA) in kidney at 6h, 12h and 24h after perfusion respectively. Results: ⑴ The degree of renal lesion in CS-HCA group was lighter than those in the other two groups at 6h after perfusion. The degree of renal lesion in both HCA group and CS-HCA group was lighter than Saline group at 12h after perfusion. It was no differences for degree of renal lesion among 3 groups at 24h after perfusion. ⑵ Comparison with HCA group and Saline group, CS-HCA group had lower AI（p<0.05）and MDA concentration（p<0.05）, and higher SOD activity（p<0.05）at 6 and 12h after perfusion. But no difference among three groups in AI and SOD activity, and MDA concentration (p>0.05）. ⑶ It was negative correlated between AI and SOD(r=-0.724), positive ratio between AI and MDA(r=0.681). Conclusions: Cordyceps artificial compound could protect the kidney against renal ischemia reperfusion injury in rats, which of mechanism may be associated with the effects of free radical and lipid peroxidation.