Abstract: Ｏｂｊｅｃｔｉｖｅ：To establish a model of uremia in pigs with high levels of proteinbound uremic toxins and observe the variation trend of relevant biological parameters in 3 months.
Ｍｅｔｈｏｄｏｌｏｇｙ：A dose of 15 g/kg·d Aristolochia manshuriensis Kom (AMK) extraction was administrated to pigs by gavage on the 1st day and 3rd day,causing acute renal injury in pigs.Subsequently,on the 4th,8th,10 th and 12th days,indoxyl and pcresol were given to pigs by gavage at 50 mg/kg·d and 100 mg/kg·d,respectively.During the experiment,relevant biological parameters were detect continuously,and pathological examinations were performed.
Ｒｅｓｕｌｔｓ：(1) Early stage(120d):  serum creatinine (SCr) and blood urea nitrogen (BUN) sharp increased，and reached the peak level to approximately 129 times(P＜001) and 99 times of the basic level (P=0011) at 815d,,together with transient proteinuria,hematuria and persistent renal tubular injury(such as urinary NAG and urinary glucose rising),indoxyl sulfate (IS) and pcresol sulfate（PCS）increased to 65 times(P＜001) and 22 times（P＜005）of the basic level on the 4th day，and further increased to 733 times and 89 times of the basic level after 4 times of indoxyl and pcresol gavage,respectively (both P<005).(2) Medium stage (2045d):characterized by partial recovery of renal function and decreased SCr,BUN,IS and PCS after reaching the peak level. (3) Late stage (4590d),there was relatively stable renal failure stage.SCr and BUN were stabilized at 62 times and 51 times of the basic level,respectively (both P<005); IS and PCS were stabilized at 125 times and 38 times of the basic level,respectively (both P<005). (4)Anemia and hyperhemocysteinemia were  also observed.(5) Renal pathological investigation showed residual tubular dilatation and interstitial collagen fiber hyperplasia 1mon after modeling,and 3mon later the lesions was more significant.
Ｃｏｎｃｌｕｓｉｏｎ：The method of AMK extraction combined with indoxyl and pcresol by gavage can quickly establish uremic model with high levels of proteinbound uremic toxins in pigs,which can be used in blood purification of proteinbound uremic toxins clearance for future research.