Abstract:

Ｏｂｊｅｃｔｉｖｅ：To study the expression of transient receptor potential melastatin 3 (TRPM3) in the internationally recognized db/db mouse model of type 2 diabetic nephropathy, in human tubular epithelial cells (HK2) and conditionally immortalized mouse podocytes (MPC) under high glucose.
Ｍｅｔｈｏｄｏｌｏｇｙ：Twenty male fiveweek db/db mice were randomly divided into two groups (each n=10), and we used db/m mice of littermate as the normal control group (n=20). At the age of 10 weeks and 18 weeks, the samples of blood, urine and renal tissues were collected. The biochemical indexes were measured. The expression of TRPM3 in renal tissues was assessed by Western blot、qRTPCR and immunohistochemistry respectively. HK2 and MPC were cultured in vitro and divided into the following groups: (1) Normal control group (Dglucose 56 mmol/L); (2) Mannitol group (Dglucose 56 mmol/L+Dmannitol 244 mmol/L); (3) High glucose group (Dglucose 30 mmol/L).The corresponding indexes were measured at 24th，48th and 72th hour. Western blot and qRTPCR were used to examine the expression of TRPM3 in protein and mRNA.
Ｒｅｓｕｌｔｓ：Compared with the 10w db/m mice, the expression of TRPM3 in the 10w db/db and 18w db/db mice was increased significantly (P<005). In cultured HK2 and MPC, the protein and mRNA expression of TRPM3 was increased in High glucose group, compared with the normal control group (P<005).
Ｃｏｎｃｌｕｓｉｏｎ：TRPM3 can be expressed in the renal tubules and glomerulus of mouse, and high glucose can induce the higher expression of TRPM3 in HK2 and MPC. TRPM3 may be involved in the development of diabetic nephropathy.