Abstract:

Ｏｂｊｅｃｔｉｖｅ：To investigate the glomerular expression of Drp1, PDrp1 (Ser616) and Fis1 in adriamycin (ADR) induced nephropathy rats and their relationship with podocyte injury.
Ｍｅｔｈｏｄｏｌｏｇｙ：ADR rat nephropathy was established. Glomerular expressions of Drp1, PDrp1 (Ser616) and Fis1 were investigated by immunohistochemistry. Renal cortical expressions of Drp1 and Fis1 were evaluated by western blot. Correlation analysis was performed to analyze the correlations between mitochondrial fission proteins, proteinuria and mitochondrial morphology. Drp1 overexpression was performed on mouse podocyte cell line, mitochondrial morphology and apoptosis were evaluated.
Ｒｅｓｕｌｔｓ：Glomerular expression of Drp1 increased at 4 and 6 weeks. Glomerular PDrp1 (Ser616) increased at 6 weeks by immunohistochemistry. Glomerular Fis1 increased at 2 and 6 weeks by immunohistochemistry. Glomerular expressions of Drp1, PDrp1 (Ser616) and Fis1 were negatively correlated with 24hour proteinuria positively. Glomerular expression of Drp1 was negatively correlated with mitochondria density in podocytes. Glomerular expression of PDrp1 (Ser616) was negatively correlated with mitochondria aspect ratio, and glomerular Fis1 was correlated with mitochondria area and circumference negatively. Drp1 overexpression led to podocyte apoptosis and mitochondrial fragmentation in MPC5.
Ｃｏｎｃｌｕｓｉｏｎ：Overexpressions of Drp1, PDrp1 (Ser616) and Fis1 participated in ADR nephropathy. Overexpression of Drp1 led to mitochondrial fragmentation and podocyte apoptosis.